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Rei Ayanami was the product of Gendo Ikari and Kozo Fuyutsuki attempt to retrieve Yui Ikari from Eva-01 using her salvaged remains. This attempt failed. On some level, Rei Ayanami may incorporate DNA from the Second Angel, Lilith, because she is the vessel for Lilith's soul. Rei Ayanami was created sometime between 2004 and 2010.